Template Switch Oligo

Template Switch Oligo - Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. However, if a large fraction of the library contains a portion. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. Use this set of oligonucleotides for template switching cdna synthesis and preamplification. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. However, if a large fraction of the library contains the. The resulting cdna can be amplified by pcr or serve as. We recommend a tso with rgrgrg at the 3′ end.

(step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. However, if a large fraction of the library contains a portion. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. The resulting cdna can be amplified by pcr or serve as. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. However, if a large fraction of the library contains the. We recommend a tso with rgrgrg at the 3′ end. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers.

(A) The TS mechanism is used for first strand cDNA synthesis. First, an

In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. The resulting cdna can be amplified by pcr or serve as. (step 2) mmlv reverse transcriptase adds.

Template Switch Oligo

In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. However, if a large fraction of the library contains the. The resulting cdna can be amplified by pcr.

Template switch and twin priming. (A) Steps describing template

However, if a large fraction of the library contains the. However, if a large fraction of the library contains a portion. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at.

Template Switching Oligo

However, if a large fraction of the library contains a portion. We recommend a tso with rgrgrg at the 3′ end. Use this set of oligonucleotides for template switching cdna synthesis and preamplification. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence.

Template Switch Oligo

In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. We recommend a tso with rgrgrg at the 3′ end. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. (step 2) mmlv reverse transcriptase adds deoxycytosines.

Fig. S12 Schematic representation of the tested oligodT / TSO

Use this set of oligonucleotides for template switching cdna synthesis and preamplification. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. The resulting cdna can be amplified by pcr or serve as. However, if a large fraction of the library contains the. In general, different tsos can.

Template switching oligos (TS oligos, TSOs) for cDNA library

In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. The resulting cdna can be amplified by.

NanoCAGE A HighResolution Technique to Discover and Interrogate Cell

In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. Use this set of oligonucleotides for template switching cdna synthesis and preamplification. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at.

Tuning 5’ to internal read proportions and template switching oligo PCR

Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. The resulting cdna can be amplified by pcr or serve as. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. However, if a large fraction of the library contains the. We recommend a tso.

(A) First strand cDNA is initiated by priming with an oligo dT primer

A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. In general, different tsos can be used including tsos with 5′ modifications, such as biotin,.

We Recommend A Tso With Rgrgrg At The 3′ End.

A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. However, if a large fraction of the library contains a portion. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. However, if a large fraction of the library contains the.

Use This Set Of Oligonucleotides For Template Switching Cdna Synthesis And Preamplification.

Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. The resulting cdna can be amplified by pcr or serve as. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2.